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You are here:Home » Antibodies » Antibodies-Collagen » Anti -Collagen Type VII

Anti -Collagen Type VII


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH IF
Type VII collagen is a newly characterized collagen which appears to be the major component of the anchoring fibril.
Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.
Catalog #C7510-66
ApplicationsSuitable for use in Electron Microscopy, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: Acetone-fixed frozen only. Fixation in methanol is not suitable.
Optimal dilutions to be determined by the researcher.
Positive ControlNormal skin
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
Clone No0.N.229
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C7510-66X.
PurityPurified by Protein G affinity chromatography.
ImmunogenInsoluble fractions of human neonatal foreskin epidermal cells. Cellular Localization
SpecificityRecognizes human intact (250kD) and collagenase digested (150kD) tissue Type VII collagen. Does not crossreact with types I, II, III, IV, V collagens, lamins or fibronectin. Epitope
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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