Anti -Collagen Type VII (BSA & Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E IH IF |
|
| Type VII collagen is a newly characterized collagen which appears to be the major component of the anchoring fibril. C7510-66 a murine monoclonal antibody to the carboxy terminal, non-collagenous region of type VII collagen. Type VII collagen, which appears to be largely restricted to epithelia of ectodermal origin including ectodermally derived glands. C7510-66 detects collagen in fetal skin early in development, from 71/2 weeks estimated gestational age. Type VII collagen can be detected in culture supernatant from epidermal keratinocytes and epithelial tumor cell lines. It does not appear to be laid down as a structured basement membrane, perhaps explaining why the cultures do not form a well defined basement membrane at the ultrastructural level. The tissue form of type VII collagen is reported to be an antiparallel dimer with overlap at the aminoterminal regions. Immunoelectron microscopy shows that all the C7510-66 binding sites are localized at the inferior border of the lamina densa. No binding could be detected within the dermis underneath the basement membrane at the putative sites of anchoring plaques. Thus C7510-66 provides an important new tool in the investigation of epithelial-mesenchymal interactions, tumorigenesis and basement membrane biology in ontogeny and epithelial differentiation. | | | Catalog # | C7510-66X | | The distribution of basement membrane type VII collagen was detected immunohistochemically and compared in normal human organs and their neoplastic derivatives using monoclonal antibody C7510-66. In normal tissues, type VII collagen was found to be restricted to the basement membrane surrounding or underlying combined epithelia, such as those lining breast, prostate, and bronchus, which are composed of a basal and luminal cell layer, and stratified epithelia, such as larynx, esophagus, trachea, vagina, ectocervix, and epidermis. No type VII collagen was found in the “simple’ epithelia lining the major part of the gastrointestinal tract (GI) tract, such as liver, stomach, and intestine, or around blood vessels, muscle, and nerve fibers, which are surrounded, however, by a basement membrane containing type IV collagen and laminin. When tested in benign and malignant local tumors, antibody LH7.2 showed staining patterns partly similar to those observed in the corresponding normal tissues. This resulted in a well-circumscribed positive reaction around ducts in carcinomas in situ of the breast, in benign prostate tumors, in pleomorphic adenomas, and in a negative reaction in tumors of the GI tract. Furthermore type VII collagen was predominantly seen in carcinomas with a squamous differentiation, such as squamous carcinomas of the lung, head and neck, vulva, and vagina. These results indicate that the presence of type VII collagen in malignant tumors is correlated with (squamous) differentiation rather than with the origin of the tumor. With tumor progression, an increased presence of type VII collagen, as compared with normal urinary bladder, was found in infiltrating transitional cell carcinomas. Thus, although in general invasive and metastatic tumors do not express extensively type VII collagen, exceptions to this rule exist in bladder cancer, squamous carcinomas of the lung, tumors of the head and neck region, female genital tract tumors, and in some adenocarcinomas of the breast. | | Applications | Suitable for use in Electron Microscopy, Immunofluorescence and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilution | Immunohistochemistry: Acetone-fixed frozen only. Not suitable for use with methanol fixation. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Normal skin | | Epitope | Located on non-helical (non-collagenous) C-terminal region of collagen VII. The epitope is resistant to collagenase, trypsin, pornase, neuraminidase, chondroitinase and hyaluronidase. It is lost by digestion of collagen VII with pepsin. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 0.N.229 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, before the addition of glycerol to 40%. Also available with BSA and azide. See C7510-66. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Insoluble fractions of human neonatal foreskin epidermal cells. Cellular Localization | | Specificity | Recognizes intact (250kD) and collagenase digested (150kD) tissue Type VII collagen. Does not crossreact with types I, II, III, IV, V collagens, lamins and fibronectin. Epitope | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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