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Collectin 11, Recombinant, Mouse (Collectin Kidney Protein 1, CL-K1, COLEC11)
Collectin 11, Recombinant, Mouse (Collectin Kidney Protein 1, CL-K1, COLEC11)
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| Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain a N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1-3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37kD collectin that circulates in the serum (4, 5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature mouse CL-K1 shares 94% and 98% amino acid sequence identity with human and rat CL-K1, respectively. Within the CRD, mouse CL-K1 shares 52% aa sequence identity with CL-L1 and 23%-32% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D. | | | Catalog # | C7549-85 | | DNA sequence encoding mouse CL-K1 (Gln 27-Leu 272; Accession # AAI0438) was fused to a 6x polyhistidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0. | | Activity | Measured by its ability to bind Alpha-L-Fucose. | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: As a result of glycosylation the recombinant mouse CL-K1 migrates as an ~33-38kD protein in SDS-PAGE under reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Molecular Weight | 27kD | | Source | Recombinant Mouse | | Purity | 85%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. | | Endotoxin | <1EU per 1ug of protein (LAL) | | Concentration | As reported | | Form | Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS to a concentration of 0.1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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