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Specifications

Catalog #	C7555-04
Applications	Suitable for use as a qualitative reference with an optimized protein content to study the normal protein composition of goat colostrum. To identify or quantitate a normal colostrum protein component using a variety of immunodiffusion techniques including immunoelectrophoresis, single and double radial immunodiffusion and electroimmunodiffusion. As a reference in nephelometry and other automated precipitation techniques. As a blocking agent or as a negative control in non-precipitating antibody-binding assays e.g. in serodiagnostic immunofluorescence and immunoenzyme tests. This normal reference does not contain assigned values for individual proteins but can be used as an internal relative standard for quantitative protein assays such as double radial immunodiffusion (Mancini, Fahey), ELISA, Western Blot and electroimmunodiffusion (Laurell), expressing the results obtained with the sample under investigation as a percentage of the protein concentration in the normal reference.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form	Supplied as a lyophilized powder. No preservative added. Reconstitute with 500ul sterile ddH2O, mix gently. Allow to stand 5-10 minutes at RT to reach equilibrium. Dilute further in PBS, pH 7.2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.