Anti -COMP (Cartilage Oligomeric Matrix Protein Precursor, EDM1, EPD1, Epiphyseal
Dysplasia 1, Epiphyseal Dysplasia 1 Multiple, MED, MGC13181, MGC149768, PSACH,
Pseudoachondroplasia, THBS5, Thrombospondin 5)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP |
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| Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110kD modular matricellular protein. COMP contains a non-collagenous coiled-coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers. An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via integrins alpha5beta1 and alphaVbeta3. COMP is upregulated in inflammation and some carcinomas. | | | | | | Applications: | | | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | | Catalog # | C7849-09 | | Recommended Dilution | Western Blot: 2ug/ml detects human COMP in cell or tissue extracts. | | Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human COMP from conditioned cell culture media. | | Optimal dilutions to be determined by the researcher. | | Western Blot Buffers | Blotting Buffer: 25mM Tris, pH 7.4, 0.15M sodium chloride, 0.05% Tween-20 | | Blocking Solution: 5% nonfat dry milk in Blotting Buffer | | Antibody Solution: 5% nonfat dry milk in Blotting Buffer | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 9H100 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Bacterially-expressed recombinant human Cartilage Oligomeric Matrix Protein (recombinant human COMP; Accession #P49747). | | Specificity | Recognizes human COMP at ~110kD. Species Sequence Homology: rat, mouse, equine, bovine, canine (86-93%) | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -COMP (Cartilage Oligomeric Matrix Protein Precursor, EDM1, EPD1, Epiphyseal Dysplasia 1, Epiphyseal Dysplasia 1 Multiple, MED, MGC13181, MGC149768, PSACH, Pseudoachondroplasia, THBS5, Thrombospondin 5) |
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