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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -COMP (Cartilage Oligomeric Matrix Protein Precursor, EDM1, EPD1, Epiphyseal Dysplasia 1, Epiphyseal Dysplasia 1 Multiple, MED, MGC13181, MGC149768, PSACH, Pseudoachondroplasia, THBS5, Thrombospondin 5)

Anti -COMP (Cartilage Oligomeric Matrix Protein Precursor, EDM1, EPD1, Epiphyseal
Dysplasia 1, Epiphyseal Dysplasia 1 Multiple, MED, MGC13181, MGC149768, PSACH,
Pseudoachondroplasia, THBS5, Thrombospondin 5)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110kD modular matricellular protein. COMP contains a non-collagenous coiled-coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers. An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via integrins alpha5beta1 and alphaVbeta3. COMP is upregulated in inflammation and some carcinomas.
Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Catalog #C7849-09
Recommended DilutionWestern Blot: 2ug/ml detects human COMP in cell or tissue extracts.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human COMP from conditioned cell culture media.
Optimal dilutions to be determined by the researcher.
Western Blot BuffersBlotting Buffer: 25mM Tris, pH 7.4, 0.15M sodium chloride, 0.05% Tween-20
Blocking Solution: 5% nonfat dry milk in Blotting Buffer
Antibody Solution: 5% nonfat dry milk in Blotting Buffer
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No9H100
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenBacterially-expressed recombinant human Cartilage Oligomeric Matrix Protein (recombinant human COMP; Accession #P49747).
SpecificityRecognizes human COMP at ~110kD. Species Sequence Homology: rat, mouse, equine, bovine, canine (86-93%)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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