You are here:Home
»
Antibodies
»
Abs to Complement
»
Anti -Complement C1rLP (C1r Like Protein, CLSPa)
Anti -Complement C1rLP (C1r Like Protein, CLSPa)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IP |
|
| Mouse C1rLP, also CLSPa, is a 55kD, secreted, atypical glycoprotein member of the AGY group of servine proteases. It has protease activity for pro-haptoglobin, but accomplishes cleavage without a typical Asp in the catalytic site. In addition, it is proposed to block unwanted complement-mediated proteolysis. Mature C1rLP is 443 amino acids (aa) in length. It contains one CUB domain (aa # 43-165), a truncated CCP2 region (aa # 167-223) and a serine protease domain (aa # 239-482). There is a potential short form that utilizes an alternate start site at Met 27. Over aa's # 44-456, mouse C1rLP is 78% and 90% aa identical to human and rat C1rLP, respectively. | | | Catalog # | C7850-07L | | Applications | Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse C1rLP. The detection limit for rmC1rLP is approximately 2 ng/lane under non-reducing and reducing conditions. | | Immunoprecipitation: This antibody has been used to immunoprecipitate rmC1rLP from conditioned media of transfected NS0 cells. | | Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse C1rLP. The detection limit for rmC1rLP is approximately 0.07 ng/well. | | Optimal dilutions should be determined by each laboratory for each application. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Mouse | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml. | | Purity | Purified by mouse C1rLP affinity chromatography. | | Immunogen | NS0-derived rmC1rLP (aa18-456) | | Specificity | Selected for its ability to recognize mouse C1rLP in direct ELISAs and Western Blots. In these formats, this antibody shows approximately 40% crossreactivity with rhC1rLP and less than 1% crossreactivity with rhC1R and rhC1S. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
