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You are here:Home » Antibodies » Abs to Complement » Anti -Complement 1s (C1s)

Anti -Complement 1s (C1s)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
C1s proenzyme is a 79.8kD glycoprotein produced primarily by hepatocytes (1) but also by other cells (2,3). The proenzyme is produced as a single-chain polypeptide of 688 aa. Upon activation the peptide is cleaved between R437 and I438 giving the activated enzyme consisting of two disulfide-linked chains, A chain (52.2kD) and B chain (27.7kD). The Ca-dependent catalytic subunit of the C1 complex (C1q, C1r, C1s) is comprised of two C1s and two C1r molecules and this serine protease tetramer is responsible for the initial steps in the classical complement pathway, cleavage of C4 and C2 (1,4,5).
Catalog #C7850-08D
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No49
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenHuman plasma C1s Proenzyme.
SpecificitySpecific for C1s proenzyme and activated free C1s and C1s in complex with C1 inhibitor. Epitope differs from that of clone 6F44. Reacts in ELISA when tested with activated C1s enzyme directly coated onto the microtiter well or in sandwich ELISA in combination with a polyclonal antibody in coat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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