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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C2

Anti -Complement C2

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Complement component C2 is a component of the classical complement pathway and is involved in activation of C3 and C5. C2 consists of a single polypeptide chain with a molecular mass of 102kD.
Catalog #C7850-09
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000
Optimal dilutions to be determined by the researcher.
HybridomaP3X63-Ag8.653 from Balb/c mice.
Culture MediumRPMI 1640 with 10% fetal calf serum.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No6F60
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenComplement component C2 isolated from human plasma (1).
SpecificitySpecific for human C2 from plasma/serum. Recognizes human C2 in both native and denatured forms. In Western Blot after SDS-PAGE this antibody reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions. In ELISA, strong reaction is seen with C2 directly coated onto the microtiter well.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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