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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C2

Anti -Complement C2

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Complement component C2 is a component of the classical complement pathway and is involved in activation of C3 and C5. C2 consists of a single polypeptide chain with a molecular mass of 102kD.
Catalog #C7850-09A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:4000
Western Blot: 1:500 After SDS-PAGE, this antibody reacts with C2 and a subfraction of C2 believed to be C2b, but only under non-reducing conditions.
Optimal dilutions to be determined by the researcher.
HybridomaX63.Ag8.653 myeloma cells with spleen cells from female CF1 x Balb/c mice.
Culture MediumRPMI 1640 with 10% fatal calf serum
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F61
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
No stabilizing proteins added.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenComplement component C2 isolated from human plasma
SpecificityRecognizes C2 from human plasma/serum. Epitope specificity differs from that of C7850-09 and C7850-09B as determined by inhibition ELISA. Reacts strongly with C2. Strong reaction is seen with human serum/plasma and when used as a biotinylated detecting antibody in sandwich ELISA in combination with a polyclonal antibody against C2. Suitable for Sepharose column purification of C2 from plasma.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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