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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Complement component C2 is a component of the classical complement pathway and is involved in activation of C3 and C5. C2 consists of a single polypeptide chain with a molecular mass of 102kD.
Catalog #	C7850-09A
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:4000
	Western Blot: 1:500 After SDS-PAGE, this antibody reacts with C2 and a subfraction of C2 believed to be C2b, but only under non-reducing conditions.
	Optimal dilutions to be determined by the researcher.
Hybridoma	X63.Ag8.653 myeloma cells with spleen cells from female CF1 x Balb/c mice.
Culture Medium	RPMI 1640 with 10% fatal calf serum
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F61
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
No stabilizing proteins added.
Purity	Purified by Protein A/G affinity chromatography from culture supernatant.
Immunogen	Complement component C2 isolated from human plasma
Specificity	Recognizes C2 from human plasma/serum. Epitope specificity differs from that of C7850-09 and C7850-09B as determined by inhibition ELISA. Reacts strongly with C2. Strong reaction is seen with human serum/plasma and when used as a biotinylated detecting antibody in sandwich ELISA in combination with a polyclonal antibody against C2. Suitable for Sepharose column purification of C2 from plasma.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.