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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Complement component C2 is a component of the classical complement pathway and is involved in activation of C3 and C5. C2 consists of a single polypeptide chain with a molecular mass of 102kD.
Catalog #	C7850-09B
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Culture Medium	Dulbecco’s modified Eagle’s medium with 10% fetal calf serum
Hybridoma	X63-Ag8.653 from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F62
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
No stabilizing proteins added.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Complement component C2 isolated from human plasma.
Specificity	Recognizes C2 from human plasma/serum. Epitope specificity differs from that of C7850-09 and C7850-09A as determined by inhibition ELISA. Reacts strongly with C2. Strong reaction is seen with human serum/plasma and no reaction is seen with serum from patients deficient in C2, when tested in sandwich ELISA with a polyclonal antibody against C2. In ELISA, reaction is also seen with C2 directly coated onto the microtiter well.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.