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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3

Anti -Complement C3

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IP IH FC IF
The complement factor C3 consists of an alpha- and a beta-chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical and mannose binding lectin activation pathways lead to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to formation of the alpha-chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues including renal epithelial cells and hepatocytes.
Catalog #C7850-10D
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 0.5ug/well
Western Blot: Reduced sample treatment and SDS-PAGE was used. Detects bands at ~110-115kD (alpha chain) and ~70 and ~80KD.
Immunofluorescence: Fix in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4uM. Pretreat with 3% hydrogen peroxide for 20 min to quench endogeneous peroxidases. Microwave in antigen unmasking solution for 2-5 min as antigen retrieval.
Immunohistochemistry (Frozen sections): fix in acetone. Antigen retrieval using 50% formic acid for 5 min or microwaved (700W) in antigen unmasking buffer.
Immunohistochemistry (paraffin): Pretreat free-floating brain tissue with 50% formic acid for 5 min. or microwave (700W) in antigen unmasking solution for 2-5min. Block endogenous persoxidase by treatment with 3% H2O2 in PBS 20 min at 25°C. Block 2hrs, 2% BSA in PBS.
Optimal dilutions to be determined by the researcher.
Positive Control MLP-lpr kidney cells
Negative Control Wild type kidney cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A525
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenC57BL/6 thymocytes
SpecificityRecognizes mouse Complement component C3 and its activation products, C3b, iC3b, C3d and C3dg.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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