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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185kD and it consists of two chains (110kD and 75kD) held together by disulfide bonds (1,2).
Catalog #	C7850-10F
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:8000
	Optimal dilutions to be determined by the researcher.
Culture Medium	RPMI 1640 with 10% fetal calf serum
Hybridoma	X63-Ag8.653
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F63
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	C3 isolated from human plasma.
Specificity	Recognizes human C3d, a fragment of the alpha chain. Epitope specificity differs from that of clones 6F64 and 6F66. Reacts with C3. Binds C3 when coated on ELISA wells or when used as detection antibody with a polyclonal C3 antibody. Reacts with C3b deposited on coated antibody molecules. In Western Blot after SDS-PAGE, reacts with C3 in both reduced and unreduced forms.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.