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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3

Anti -Complement C3

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185kD and it consists of alpha and beta chains (110kD and 75kD respectively) held together by disulfide bonds.
Catalog #C7850-10G
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:4000
Western Blot: 75kD beta-chain band on SDS-PAGE Western Blot of reduced C3 and 20kD and 17kD beta-chain fragments produced by cyanogen bromide cleavage
Optimal dilutions to be determined by the researcher.
HybridomaX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No6F64
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenC3 isolated from human plasma.
SpecificityRecognizes an allotypic marker on human C3 (Leu instead of Pro in codon 314 of exon 9), which occurs regulsrly in the beta-chain of C3F and occasionally in the beta-chain of C3S.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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