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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185kD and it consists of two chains (110kD and 75kD) held together by disulfide bonds.
Catalog #	C7850-10H
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	Western Blot: Reducing and non-reducing gels
	Optimal dilutions to be determined by the researcher.
Culture Medium	RPMI 1640 with 10% fetal calf serum
Hybridoma	X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F65
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity	Purified by Protein A/G affinity chromatography
Immunogen	C3 isolated from human plasma.
Specificity	Recognizes human Complement component C3.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.