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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3

Anti -Complement C3

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185kD and it consists of two chains (110kD and 75kD) held together by disulfide bonds.
Catalog #C7850-10H
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: Reducing and non-reducing gels
Optimal dilutions to be determined by the researcher.
Culture MediumRPMI 1640 with 10% fetal calf serum
HybridomaX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F65
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography
ImmunogenC3 isolated from human plasma.
SpecificityRecognizes human Complement component C3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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