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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3

Anti -Complement C3

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185kD and it consists of two chains (110kD and 75kD) held together by disulfide bonds.
Catalog #C7850-10J
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:15,000
Optimal dilutions to be determined by the researcher.
HybridomaX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Culture MediumDulbecco’s modified Eagle’s medium with 10% fetal calf serum.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F66
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenC3 isolated from human plasma.
SpecificityRecognizes human C3d, a fragment of the alpha chain. Epitope specificity differs from that of clones 6F63, 6F64, and 6F65 but overlaps slightly as determined by inhibition ELISA. Reacts strongly with C3. Strong reaction is seen in ELISA with C3 directly coated onto the microtiterwell, or when used as detection antibody in sandwich ELISA in combination with a polyclonal antibody against C3. In Western Blot after SDS-PAGE, this antibody reacts with C3 in reduced as well as nonreduced forms.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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