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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3

Anti -Complement C3

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
C3 is a key component of the complement system since the classical and the alternative activation pathways merge at the C3 activation step. The innate immune system including the complement system was the first to emerge before adaptive immune responses evolved, thus the components involved are very similar in physological different species.
Catalog #C7850-10K
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:2000
Optimal dilutions to be determined by the researcher.
HybridomaX63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.
Culure medium RPMI 1640 with 10% fetal calf serum
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F67
HostMouse
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenC3 purified from rat serum.
SpecificityRecognizes the complement component of rat C3. No cross-reaction is seen when tested with human C3.
Reacts strongly with rat C3. Strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal antibody against rat C3. Administered to rats transplanted with guinea-pig hearts it prolongs xenograft survival.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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