| The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectinactivation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further |
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| degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes. |
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| Catalog # | C7850-10P |
| Applications | Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA: 1-2ug/ml |
| Immunocytochemistry: 1:200 |
| Western Blot: 1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906: IgY, Chicken Pab Rb x Ch |
| I1906-10: IgY, Chicken (HRP) Rb x Ch |
| I1906-12: IgY, Chicken (FITC) Rb x Ch |
| I1906-14: IgY, Chicken (AP) Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Human |
| Concentration | ~1.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2. No preservative added. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Full length human plasma protein. Cellular localization: Secreted |
| Specificity | Recognizes human Complement C3 at ~187kD. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | Complement component 3, ASP, Complement C3 precursor, CPAMD1, Acylation Stimulating Protein Cleavage Product |
