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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3 (C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1, C3, CPAMD1) (Biotin)

Anti -Complement C3 (C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1,
C3, CPAMD1) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Human Complement component 3, C3 (alternative names include acylation-stimulating protein (ASP) C3 is encoded by gene located 19p13.3-p13.2. Because C3, C4, and C5 are strikingly similar suggesting a common evolutionary origin. C3 is an acute phase reactant. Synthesis of C3, a glycoprotein, is induced during acute inflammation. The liver is the main site of synthesis, although small amounts are also produced by activated monocytes and macrophages. A single chain precursor (pro-C3) of approximately 200 kD is found intracellularly; the cDNA shows that it comprises 1,663aa. This is processed by proteolytic cleavage into alpha (~115kD) and beta subunits (~75kD) which in the mature protein are linked by disulfide bonds. Pro-C3 contains a signal peptide of 22aa residues, the beta chain (645 residues) and the alpha chain (992 residues). The 2 chains are joined by 4 arginine residues that are not present in the mature protein. Human C3 has 79% identity to mouse C3 at the nucleotide level and 77% at the aa level.
Catalog #C7850-12G
Human C3 concentration in normal human serum is ~1.25mg/ml. Classical and alternative activation pathways of complement converge at C3 step. Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces. Both C3 convertases cleave the C3 a-chain at peptide bond 77 resulting in production of C3a (Molecular Weight 9083) and C3b fragments (Molecular Weight 80,000). Released C3a peptide is one of the three complement anaphylatoxins. The nascent C3b fragment can form a covalent ester bond with target surface. This covalent attachment of C3b to target acceptors is required for continuation of complement activation.
C3 nephritic factor, an IgG antibody against complement components, is demonstrable in some cases of partial lipodystrophy. C3-deficienthomozygotesdeveloped mesangiocapillary glomerulonephritis.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:5000-1:30,000
Western Blot: 1:2000 1:10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceCanine
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. Labeled with Biotin. F/P ratio
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly purified canine serum C3 protein.
SpecificityRecognizes canine C3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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