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Anti -Complement C3 (C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1, C3, CPAMD1)
Anti -Complement C3 (C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1,
C3, CPAMD1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Serum |
E B |
|
| Human Complement component 3, C3 (alternative names include acylation-stimulating protein (ASP) C3 is encoded by gene located 19p13.3-p13.2. Because C3, C4, and C5 are strikingly similar suggesting a common evolutionary origin. C3 is an acute phase reactant. Synthesis of C3, a glycoprotein, is induced during acute inflammation. The liver is the main site of synthesis, although small amounts are also produced by activated monocytes and macrophages. A single chain precursor (pro-C3) of approximately 200kD is found intracellularly; the cDNA shows that it comprises 1663aa. This is processed by proteolytic cleavage into alpha (~115kD) and beta subunits (~75kD) which in the mature protein are linked by disulfide bonds. Pro-C3 contains a signal peptide of 22aa residues, the beta chain (645 residues) and the alpha chain (992 residues). The 2 chains are joined by 4 arginine residues that are not present in the mature protein. Human C3 has 79% identity to mouse C3 at the nucleotide level and 77% at the amino acid level. | | | Catalog # | C7850-12M | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:1000-1:5000 (ECL) | | ELISA: 1:10,000-50,000 (50-100ng control antigen/well) | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS. | | Purity | Serum | | Immunogen | Highly purified human serum C3 protein. | | Specificity | Recognizes human C3 protein. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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