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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3, C3b

Anti -Complement C3, C3b

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. An inherited deficiency of C3 predisposes a person to frequent bacterial infections. C3 fragments are deposited in tissues at sites of antibody-mediated immunopathology. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. After activation of the complement system, certain enzymes become active, resulting in the cleavage of C3 into C3b and the anaphylatoxin C3a. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. Within the alternative pathway of complement, C3b plays a critical role in the amplification loop initiated by spontaneous hydrolysis of C3.
Catalog #C7850-13E
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot : 1:50 detects a band at ~100kd (reduced) and at ~190kD (non-reduced)
Optimal dilutions to be determined by the researcher.
Positive ControlHuman serum
Negative Control:
C3 deficient serum
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A528
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenNative human C3
SpecificityRecognizes human C3 and C3b. Recognizes an epitope located in the C-terminal 360 amino acids (mapped to aa1499-1519) on the alpha chain of human C3. Does not react with C3a.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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