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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3a, C3a des-Arg, Human

Anti -Complement C3a, C3a des-Arg, Human

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg).
Catalog #C7850-13H
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:16,000
Optimal dilutions to be determined by the researcher.
Matched PairCapture: C7850-13H
Dectection: C7850-13J
HybridomaBalb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F68
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenHuman C3a.
SpecificityRecognizes an epitope that is present on human C3, C3a and C3a (desArg). Does not cross-react with C4a or C5a. Recognizes a different epitope on the 9kD C3a than C7850-13J. Effectively inhibits the biological activitiy of C3a in a guinea-pig platelet activation assay.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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