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Anti -Complement C3a, C3a des-Arg, Human
Anti -Complement C3a, C3a des-Arg, Human
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg). | | | Catalog # | C7850-13J | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:32,000 | | Optimal dilutions to be determined by the researcher. | | Recommended Pair | Capture: C7850-13H | | Dectection: C7850-13J | | Hybridoma | Balb/c mice | | Culture Medium | RPMI 1640 with 10% fetal calf serum. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 6F69 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in 0.01M PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%. | | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Human C3a | | Specificity | Recognizes an epitope that is present on human C3, C3a and C3a (desArg). Does not crossreact with C4a or C5a. Recognizes different epitopes on the 9kD C3a than C7850-13H. No reaction is observed with a synthetic octapeptide representing the C3a C-terminal. Does not inhibit the biological activity of C3a. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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