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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3a, C3a des-Arg, Human

Anti -Complement C3a, C3a des-Arg, Human

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg).
Catalog #C7850-13J
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Optimal dilutions to be determined by the researcher.
Recommended PairCapture: C7850-13H
Dectection: C7850-13J
HybridomaBalb/c mice
Culture MediumRPMI 1640 with 10% fetal calf serum.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F69
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.01M PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenHuman C3a
SpecificityRecognizes an epitope that is present on human C3, C3a and C3a (desArg). Does not crossreact with C4a or C5a. Recognizes different epitopes on the 9kD C3a than C7850-13H. No reaction is observed with a synthetic octapeptide representing the C3a C-terminal. Does not inhibit the biological activity of C3a.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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