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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E

C3b (C3c+C3d) has a molecular weight of 170000.
Catalog #	C7850-13U
Applications	Suitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested.
Recommended Dilution	Immunoelectrophoresis: 120ul against 2ul human plasma or equivalent.
	Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells.
	Single Radial Immunodiffusion: 1% in gel
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a lyophilized powder. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Purity	Serum
Immunogen	C3b isolated and purified from pooled normal human serum.
Specificity	Recognzies the C3b subunit of human complement C3. In immunoelectrophoresis and radial immunodiffusion against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. Does not react with any other protein component of human serum or plasma.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.