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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3b, Human

Anti -Complement C3b, Human

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E
C3b (C3c+C3d) has a molecular weight of 170,000.
Catalog #C7850-13U
ApplicationsSuitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested.
Recommended DilutionImmunoelectrophoresis: 120ul against 2ul human plasma or equivalent.
Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells.
Single Radial Immunodiffusion: 1% in gel.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
PuritySerum
ImmunogenC3b isolated and purified from pooled normal human serum.
SpecificityRecognzies the C3b subunit of human complement C3. In immunoelectrophoresis and radial immunodiffusion against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. Does not react with any other protein component of human serum or plasma.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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