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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH

The antibody allows demonstration of C3 deposition in the tissue or on cells. It should be used in combination with an additional antibody to the C3 alpha chain (positive control) and an antibody to a determinant on C3a (negative control). Activated, deposited C3b bears the C3b alpha-chain and beta-chain determinants but no longer the C3a determinants, whereas ubiquitous or non-specifically absorbed C3 is still C3a-positive.
Catalog #	C7850-14
Applications	Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry: Frozen sections
	Optimal dilutions to be determined by the researcher.
Source	Ascites
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8.B.70
Host	Mouse
Source	Human
Concentration	~0.05mg/ml
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human complement component C3
Specificity	Recognizes a determinant present on native human (circulating) C3 and on the fragments C3b and C3c. The antibody is directed to a determinant present on the beta-chain of the C3-molecule as determined by Western Blot. Does not crossreact with anaphylatoxin C3a or fragment C3d. Does not inhibit the hemolytic function of C3.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.