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Specifications

Clone Host Grade Applications Polyclonal Porcine Purified E

Human Complement component 3, C3 (alternative names include acylation-stimulating protein (ASP) C3 is encoded by gene located 19p13.3-p13.2. Because C3, C4, and C5 are strikingly similar suggesting a common evolutionary origin. C3 is an acute phase reactant. Synthesis of C3, a glycoprotein, is induced during acute inflammation. The liver is the main site of synthesis, although small amounts are also produced by activated monocytes and macrophages. A single chain precursor (pro-C3) of approximately 200kD is found intracellularly; the cDNA shows that it comprises 1,663aa. This is processed by proteolytic cleavage into alpha (~115kD) and beta subunits (~75kD) which in the mature protein are linked by disulfide bonds.
Catalog #	C7850-14C6
Applications	Suitable for use in ELISA, Immunoelectrophoresis and single and double radial immunodiffusion. Other applications not tested.
Recommended Dilution	Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserum
	Double Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mm
	diameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameter
	peripheral wells.
	Single Radial Immunodiffusion: 1% in the gel.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Porcine
Source	Human
Concentration	Not determined
Form	Supplied as a lyophilized powder in PBS, pH 7.2. Preservative free. Reconstitute with 1ml sterile dH2O.
Purity	Purified
Immunogen	C3 cleavage by C3 convertase and factor I. C3c is isolated and purified from pooled normal human serum.
Specificity	Recognizes human C3c.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.