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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B

Catalog #	C7850-14F
Source	Mouse ascitic fluid
Applications	Suitable for use in Immunohistochemistry (frozen sections) and Western blotting. The antibody is, in combination with antibodies to the C3b fragment, an excellent control for distinguishing ubiquitous or nonspecifically bound native C3 from the biologically active C3 fragment. Nonactivated C3 still bears the C3a determinant, whereas activated C3 (C3b, C3d) is C3a-negative. It is therefore useful for immunopathological assays (demonstration of C3 deposition in biopsies) and for C3a assays research purposes.
Recommended Dilution	Optimal dilution determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	2Q2126
Host	Mouse
Source	Human
Concentration	Not determined
Form	Lyophilyzed. Reconstitute with 1ml distilled water.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human complement component C3
Specificity	Recognizes a determinant on the alpha chain of C3 (Western blot). The determinant is localized in the C3a-segment of the alpha chain. The antibody inhibits the anaphylactic function of C3a
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.