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You are here:Home » Antibodies » Antibodies-Complement » Anti -Complement C3a, Human

Anti -Complement C3a, Human


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
The antibody is, in combination with antibodies to the C3b fragment, an excellent control for distinguishing ubiquitous or nonspecifically bound native C3 from the biologically active C3 fragment. Nonactivated C3 still bears the C3a determinant, whereas activated C3 (C3b, C3d) is C3a-negative. It is therefore useful for immunopathological assays (demonstration of C3 deposition in biopsies) and for C3a assays.
Catalog #C7850-14F
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen): 1:10. One hour incubation at RT.
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No2Q2126
Concentration~0.05mg/ml (after reconstitution)
FormSupplied as a lyophilyzed powder from PBS, pH 7.4, 0.5% BSA, 0.9% sodium azide. Reconstitute with 1ml sterile ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman complement component C3
SpecificityRecognizes an epitope on the human C3 alpha,-chain, or C3a which is present on native C3. The antibody inhibits the anaphylactic function of C3a
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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