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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3b beta, Human

Anti -Complement C3b beta, Human

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. Proteolysis by certain enzymes results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. Furthermore, C3b plays a critical role in the amplification loop of the alternative pathway.
Catalog #C7850-14G
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen): 1:10. 1hr at RT
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F202
HostMouse
SourceHuman
Concentration~0.05mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman complement component C3.
SpecificityRecognizes human complement component C3b beta. Allows demonstration of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. Does not react with C3a or C3d. Inhibits hemolytic function of C3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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