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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1) (Rhodamine)

Anti -Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein
Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1) (Rhodamine)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Purified E
C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain.
Catalog #C7850-14H7
ApplicationsSuitable for use in FLISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Excitation 554nm
Emission 573nm
Molar Ratio ~1.4
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Rhodamine conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with Rhodamine. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
PurityPurified
ImmunogenC3c isolated and purified from pooled normal human serum.
SpecificityRecognizes human C3c. In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single
precipitin line in the alpha-2 region is obtained. Can also react with fragments C3b, C3bi and
smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of human serum or plasma.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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