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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, AHUS5, ARMD9, C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1, Complement Component 3, Complement Factor 3, CPAMD1) (FITC)

Anti -Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein
Cleavage Product, ASP, AHUS5, ARMD9, C3 and PZP-like alpha-2-macroglobulin
Domain-containing Protein 1, Complement Component 3, Complement Factor 3, CPAMD1) (FITC)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Purified
C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is
composed of an intact beta chain bound to two fragments of the alpha chain.
Catalog #C7850-14P4
ApplicationsSuitable for use in FLISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Fluorescein/IgG protein molar ratio (F/P) ~1.9
Excitation 492nm
Emission 515nm
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMonkey
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with fluorescein isothiocyanate (FITC). Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
PurityPurified
ImmunogenC3c isolated and purified from pooled normal monkey serum.
SpecificityRecognizes monkey C3, C3c. In immunoelectrophoresis against fresh monkey serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Also reacts with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of monkey serum or plasma.Species Crossreactivity: Old world monkeys (Cercopithecus, Cynomolgus and Baboon), chimpanzee, human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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