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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum

C3 is the most abundant complement protein in bovine serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common in both pathways. Activation of C3 is achieved by specific limited proteolysis resulting in the increase of a number of degradation fragments. The anaphylatoxin C3a promotes smooth muscle contraction and increases vascular permeability; the large C3b fragment is involved in binding to the complement activator and can interact with specific receptors to allow efficient clearance of the activating cell or particle; degradation fragments of C3b (C3bi, C3c, C3dg and C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possible in the immune response.
The antiserum is raised against C3c which is the major fragment resulting from the C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Consequently antisera to C3c react with both native and activated C3. It may also react with the fragments C3b, C3bi and C3dg, since they all carry antigenic epitopes of the C3c domain. The protein is isolated and purified from pooled normal bovine serum by precipitation techniques, followed by chromatographical methods.
Catalog #	C7850-14P8
Applications	Suitable for use in IEP, Single and Double RID. Other applications have not been tested.
Recommended Dilutions
	IEP: use 2ul serum, plasma or equivalent against 120ul antiserum.
	Double RID: Use a rosette arrangement with 10ul antiserum in a 3 mm diameter centre well and 2ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
	SIngle RID: use 1 percent antiserum in the gel.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Bovine
Concentration	Not determined
Form	Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O. Dilute further with PBS, pH 7.2.
Purity	Serum
Immunogen	Complement C3, C3c subunit
Specificity	Recognizes bovine Complement C3, C3c subunit.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.