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Anti -Complement C3, C3c Subunit
Anti -Complement C3, C3c Subunit
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
|
|
| C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is composed of an intact beta chain bound to two fragments of the alpha chain. | | | Catalog # | C7850-14S9 | | Applications | Suitable for use in IEP, Single and Double RID. Other applications have not been tested. | | Recommended Dilutions | | | IEP: use 2ul serum, plasma or equivalent against 120ul antiserum. | | Double RID: Use a rosette arrangement with 10ul antiserum in a 3 mm diameter centre well and 2ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. | | SIngle RID: use 1% antiserum in the gel. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Guinea pig | | Concentration | Not determined | | Form | Supplied as a lyophilized powder. No preservatives added. Reconstitute with 1ml sterile ddH2O. Dilute further with PBS, pH 7.2. | | Purity | Serum | | Immunogen | The protein is isolated and purified from pooled normal guinea pig serum by precipitation techniques, followed by chromatographical methods. | | Specificity | Recognizes Guinea pig Complement C3, C3c subunit. In IEP against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of mouse serum or plasma. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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