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Specifications

Clone Host Grade Applications Polyclonal Goat Serum E

C4 and C2 represent substrates for activated C1. Both components are present in serum in an
inert form. C4 is composed of 3 polypeptide chains of three different types, a, b, g. One of the
chains is cleaved by the action of activated C1, resulting in activation of C4 exposing an binding
site for the next component in the sequence, C2. C4 has a molecular weight of 205000 and is
present in plasma in an average concentration of 600mg/ml. The activation of C4 yields two
peptide fragments: C4a (MW 8000) and C4b (MW 198000). This process shows a considerable degree of similarity to the activation of the components C3 and C5.
Catalog #	C7850-15E
Applications	Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	Not determined
Form	Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Purity	Serum
Immunogen	Human C4 isolated as a homogenous protein
Specificity	Recognizes human C4. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony) against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C4 protein. Activated C4b may also react with the antiserum. No reaction is obtained with any other plasma protein component or serum.
Does not crossreact with any other component of human plasma.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.