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Specifications

Clone Host Grade Applications Polyclonal Goat Serum E

C4 consists of three polypeptide chains (alpha, molecular weight 95kD, beta, molecular weight 75kD and gamma, molecular weight 33kD) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.
Catalog #	C7850-16H
Applications	Suitable for use in ELISA. Other applications not tested
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Goat
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 0.05M tris, 0.5M sodium chloride, pH 7.5, 0.05% sodium azide.
Purity	Purified by delipidation followed by solid phase absorption and sterile-filtration (Turbidometric grade).
Immunogen	Purified human C4.
Specificity	Recognizes human Complement C4.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.