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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Complement factor C4, formerly known as Gg protein, consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved resulting in C4c and C4d, in which step all functional sites are lost. Therefore, the C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies.
Catalog #	C7850-18G
Applications	Suitable for use in Western Blot, Immunohistochemistry (frozen), and Immunoassays. Other applications not tested.
Recommended Dilution	Western Blot: 1:50
	Immunohistochemistry (frozen): 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9G175
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity	Purified
Immunogen	Human C4d.
Specificity	Recognizes an epitope on the alpha 2 chain of human C4d. It recognizes C4, C4b and C4d. Species crossreactivity: guinee pig
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.