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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C5, Human

Anti -Complement C5, Human

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
C5 component of human complement is a plasma protein consisting of two polypeptide chains,
a, b, bound by disulphide bonds. It has a molecular weight of 190000. The activated protein forms with the complement factors C6-C9 the membrane attack complex. The average concentration in plasma is about 70mg/ml.
Catalog #C7850-23
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen): 1:10; Incubation Time 1hr at RT.
Optimal dilution to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F207
HostMouse
SourceHuman
Concentration~0.05mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman complement component C5.
SpecificityRecognizes human native C5 as well as with C5b. Allows detection of native complement component C5 and discrimination from activated C5b-9 complexes, especially in different forms of glomerulonephritis.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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