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You are here:Home » Antibodies » Antibodies-Complement » Anti -Complement C5

Anti -Complement C5


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9) (1). The molecular mass of C5 is 190kD.
Catalog #C7850-23A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Culture MediumRPMI 1640 with 10% fetal calf serum.
HybridomaX63-Ag8.653. Female CF1 x Balb c mice immunized by intraperitoneal injection.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No6F72
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide
PurityPurified by Protein A affinity chromatography.
ImmunogenC5 isolated from human plasma.
SpecificityRecognizes human C5. Epitope specificity differs from that of clone 11F6 but slightly overlaps as determined by inhibition ELISA. Reacts strongly with C5. No reaction seen from patients deficient in C5, when tested in sandwich ELISA in combination with a polyclonal antibody against C5. In Western Blot after SDS-PAGE, reacts with C5 in normal human plasma (PEG precipitated C5) in nonreduced form only, no reaction from C5 deficient patients.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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