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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9) (1). The molecular mass of C5 is 190kD.
Catalog #	C7850-23A
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:4000
	Optimal dilutions to be determined by the researcher.
Culture Medium	RPMI 1640 with 10% fetal calf serum.
Hybridoma	X63-Ag8.653. Female CF1 x Balb c mice immunized by intraperitoneal injection.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F72
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	C5 isolated from human plasma.
Specificity	Recognizes human C5. No reaction from patients deficient in C5. Epitope specificity differs from that of clone 6F73 but slightly overlaps as determined by inhibition ELISA. Reacts strongly with C5. No reaction seen from patients deficient in C5, when tested in sandwich ELISA in combination with a polyclonal antibody against C5. In Western Blot after SDS-PAGE, reacts with C5 in normal human plasma (PEG precipitated C5) in nonreduced form only, no reaction from C5 deficient patients.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.