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You are here:Home » Antibodies » Antibodies-Complement » Anti -Complement C5

Anti -Complement C5


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
C5 is part of the membrane attack complex (MAC) of the complement system. C5 is split into C5a og C5b by the C5 convertases from the classical and the alternative complement pathway and the C5b fragment initiates the assembly of the terminal MAC (C5b-C9) (1). The molecular mass of C5 is 190kD.
Catalog #C7850-23B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No11F6
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenC5 isolated from human plasma.
SpecificityRecognizes human C5. No reaction is seen with plasma from patients deficient in C5. Reacts strongly with C5. Strong reaction is seen with normal human plasma, and no reaction is seen with plasma from patients deficient in C5, when tested in sandwich ELISA in combination with a polyclonal antibody against C5. In Western Blot, after SDS-PAGE this antibody reacts with C5 in normal human plasma (PEG precipitated C5) in nonreduced form only. No reaction is seen with plasma from C5 deficient patients.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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