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You are here:Home » Antibodies » Antibodies-Complement » Anti -Complement C5, Human

Anti -Complement C5, Human


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kD glycoprotein consisting of a disulphide linked alpha-chain (115kD) and beta-chain (75kD). C5 occurs in plasma at about 75mg/L. It is activated via either the classical or the alternative complement pathway, by protoelytic cleavage of C5a (11.5kD) from the N-terminal of the alpha-chain. C5a is a potent anaphylotoxin and stimulates directed migration of neutrophils, eosinophils, basophils and monocytes. The other fragment, C5b,initiates the assembly of the membrane attack complex that mediates the cytolysis of viral and bacterial pathogens.
Catalog #C7850-24M
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:100-1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No8J448
FormSupplied as a liquid in PBS, 1% BSA, 0.02% sodium azide
PurityPurified by Protein G affinity chromatography.
SpecificityRecognizes an epitope on C5 and on C5b at ~188kD. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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