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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B

C5 is synthesized in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kD glycoprotein consisting of a disulphide linked alpha-chain (115kD) and beta-chain (75kD). C5 occurs in plasma at about 75mg/L. It is activated via either the classical or the alternative complement pathway, by protoelytic cleavage of C5a (11.5kD) from the N-terminal of the alpha-chain. C5a is a potent anaphylotoxin and stimulates directed migration of neutrophils, eosinophils, basophils and monocytes. The other fragment, C5b,initiates the assembly of the membrane attack complex that mediates the cytolysis of viral and bacterial pathogens.
Catalog #	C7850-24M
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	8J448
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 0.02% sodium azide, 1% BSA.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Complement C5
Specificity	Recognizes an epitope on C5 and on C5b at ~188kD. Species Crossreactivity: human
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.