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Specifications

Clone Host Grade Applications Polyclonal Goat Serum E

C5 component of human complement is a plasma protein consisting of two polypeptide chains,
a, b, bound by disulphide bonds. It has a molecular weight of 190000. The activated protein forms with the complement factors C6-C9 the membrane attack complex. The average concentration in plasma is about 70mg/ml.
Catalog #	C7850-24U1
Applications	Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.
Recommended Dilution	Immunoelectrophoresis: 120ul against 2ul or equivalent
	Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in a 3mm diameter center well and 2ul serum samples (neat and serially diluted) in 2mm diameter peripheral wells.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	Not determined
Form	Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Purity	Serum
Immunogen	Human C5 isolated as a homogenous protein
Specificity	Recognizes human C5. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony) against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C5 protein. No reaction is obtained with any other plasma protein component or serum.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.