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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C9

Anti -Complement C9

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Complement factor C9 is a 71kD single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and have an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). Primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60ug/ml, and up-regulated from hepatocytes by IFN-g.
Catalog #C7850-52
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:30,000 Recognizes C9 in human seum diluted 1:50 in Tris buffer (20mM Tris-base, 1mM magnesium chloride, 1mM calcium chloride, 140mM sodium chloride) and incubated for 2 hours at 37ºC using a human IgM coated (10ug/ml overnight at 4ºC, blocked with PBS, pH 7.2, 1% BSA for 1 hour).
Optimal dilutions to be determined by the researcher.
Culture MediumRPMI 1640 with 2-10% fetal calf serum
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F74
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenHuman complement factor C9.
SpecificityRecognizes human complement C9.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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