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You are here:Home » Antibodies » Antibodies-Complement » Anti -Complement Factor B

Anti -Complement Factor B


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Factor B is a serine protease of the alternative pathway of the complement system. The amino acid sequence of human factor B is 85% identical to that of rat and mouse.
Catalog #C7850-60A
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~5ng/lane under non-reducing conditions. Use of reducing conditions is not recommended.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human Factor B from conditioned cell culture medium.
Direct ELISA: 0.5-1ug/ml; The detection limit is ~2ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No7H80
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa26-764 of human Factor B expressed in NS0 cells (Accession # P00751).
SpecificityRecognizes recombinant human Complement Factor B. Does not crossreact with recombinant human Factor D or recombinant human Factor I. Species Sequence Homology: rat and mouse, 85%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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