Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry (frozen), and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Immunohistochemistry Positive Control
Kidney from post streptococcal glomerulonephritis patients
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human factor B
Form
Supplied as a liquid in borate buffered saline containing 0.09% sodium azide
Purity
Purified by Protein A affinity chromatography
Specificity
Recognizes the Bb fragment of human complement factor B. This antibody binds to both the Bb fragment and complement factor B so is not suitable for all ELISA applications.
References
1. Xu, Y. et al. (2001) Structural biology of the alternative pathway convertase. Immunol. Rev. 180:123-135, 2. Ponnuraj, K. et al. (2004) Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase. Mol. Cell. 14:17-28.
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