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You are here:Home » Antibodies » Abs to Complement » Anti -Complement Factor D

Anti -Complement Factor D

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Complement factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25kD. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.
Catalog #C7850-70
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: Used as a biotinylated detection antibody in sandwich ELISA with C7850-71 as the capture antibody(1).
Western Blot: Can be used following SDS-PAGE under non-reducing conditions (2).
Optimal dilutions to be determined by the researcher.
Culture MediumRPMI 1640 with 10% fetal calf serum
HybridomaX63-Ag8.653 from BALB/c mice immunized ip.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F82
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenHuman factor D (1).
SpecificityRecognizes human Complement Factor D in serum and plasma. Epitope specificity differs from that of C7850-71.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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