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You are here:Home » Antibodies » Abs to Complement » Anti -Complement Factor D

Anti -Complement Factor D

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IP
Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).
Catalog #C7850-70D
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 0.1ug/ml; Recombinant human Complement Factor D/Adipsin
Immunoprecipitation: 0.25ug/ml; Conditioned cell culture medium spiked with Recombinant human Complement Factor D/Adipsin
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, Trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenMouse myeloma cell line NS0-derived recombinant human Complement Factor D Ile26-Ala253 Accession # P00746
SpecificityRecognizes human Complement Factor D in direct ELISAs and Western Blots. In direct ELISAs, less than 1% crossreactivity with recombinant human (rh) Complement Factor B and rhComplement Factor I is observed.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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