| Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3). |
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| Catalog # | C7850-70E |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Sandwich ELISA: |
| Capture Antibody: 2-8ug/ml, Human Complement Factor D/Adipsin Antibody as detection (Catalog #C7850-70C) |
| Detection Antibody: 0.1-0.4ug/ml; Human Complement Factor D/Adipsin Biotinylated Antibody (Catalog #C7850-70E) |
| ELISA Sample: Recombinant Human Complement Factor D/Adipsin |
| Western Blot: 0.1ug/ml; Recombinant human Complement Factor D/Adipsin |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~0.1mg/ml (After reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, BSA. Reconstitute with 500ul sterile PBS. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Complement Factor D Ile26-Ala253, Accession # P00746 |
| Specificity | Recognizes human Complement Factor D in ELISAs and Western Blots. In sandwich immunoassays, less than 0.1% crossreactivity with rhFactor II, rhFactor X, and rhFactor XI is observed. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
