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You are here:Home » Antibodies » Abs to Complement » Anti -Complement Factor D (Biotin)

Anti -Complement Factor D (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3. Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity.
Catalog #C7850-70E
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsSandwich ELISA:
Capture (C7850-70C): 2-8ug/ml
Detection(C7850-70E): 0.1-0.4ug/ml
Western Blot: 0.1ug/ml using Recombinant human Complement Factor D/Adipsin
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa26-253 from human Complement Factor D expressed in NSO cells (P00746).
SpecificityRecognizes human Complement Factor D in ELISAs and Western Blots. In sandwich immunoassays, <0.1% crossreactivity with rhFactor II, rhFactor X and rhFactor XI.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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