| Intercellular communication through gap junctions plays an important role in a variety of cellular processes including |
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| homeostasis, morphogenesis, cell differentiation, and growth control. Gap junctions are transmembrane channels that |
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| serve to directly link neighboring cells by mediating the exchange of low-MW <1200 kD metabolites, ions, and second messengers. Gap junctions are formed by the interaction of hemichannels (connexons) on adjacent cells. The |
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| connexon itself is composed of a hexameric assembly of connexin proteins. Connexins are highly homologous proteins |
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| encoded by a multigene family. The connexins exhibit similar structural features, which include a cytoplasmic amino terminal |
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| region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length. |
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| Catalog # | C7853-04 |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA (Immunogen): 0.1-1.0ug/ml |
| Immunohistochemistry (Rat): 10-20ug/ml |
| Western Blot (Mouse and Rat): 1-2ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Mouse |
| Concentration | ~0.25mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to 13aa derived from the C-terminus of the mouse Connexin 26 protein. This mouse sequence differs from the rat sequence by a single aa and from the human sequence by two (non-consecutive) amino acids. |
| Specificity | Recognizes mouse Connexin 26. Species Crossreactivity |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
