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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 26 (CX26, Gap Junction Beta 2 Protein, CXB-2)

Anti -Connexin 26 (CX26, Gap Junction Beta 2 Protein, CXB-2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Intracellular communication mediated by gap junctions plays an important role in a variety of cellular processes including: homeostasis, morphogenesis, cell differentiation, and growth control. Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of low-molecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family. The connexins exhibit similar structural features which include a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxyl-terminal cytoplasmic tail of varying length. Comparison of the aa sequences of the various connexin family members indicate that the two areas of greatest divergence amongst the connexin family members are the intracellular loop connecting the second and third transmembrane segments and the carboxy-terminal tail. These domains are, therefore, thought to mediate connexin-type speci c properties including: phosphorylation, responses to gating stimuli, as well as assembly and membrane turnover. Modulation of gap junctional communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, postranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis. Mouse connexin 26 is a 226aa gap junction protein with a predicted molecular weight of ~26kD. It is prominently expressed in liver.
Catalog #C7853-05A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000
ELISA: 1:100,000 in 50-100ng C7853-15 Connexin 26, Mouse, Control Peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in 0.05% sodium azide, 40% glycerol.
PuritySerum
ImmunogenSynthetic peptide corresponding to 19aa near the C-terminal, 2nd cytoplasmic domain of the mouse Cx26 (KLH).
SpecificityRecognizes mouse Connexin 26. Species sequence homology: rat-100%, human- 90%, sheep-85%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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