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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3)

Anti -Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IF
Connexin 31 (Cx31) is a 270 amino acid gap junction protein in mouse and rat with a predicted molecular weight of ~31kD.1 Connexin 31 is encoded by the gene Gjb3, and is expressed in a complex pattern throughout mouse development. During early mouse development, Cx31 is present in both the inner cell mass and the trophectoderm, but it is restricted to cells derived from the trophectoderm lineage after implantation. In adult female rodents, Cx31 is expressed in the granular layer of the placenta, and the lack of Cx31 leads to transient dysmorphogenesis affecting embryonic survival. In adult male rodents, Cx31 is expressed in the seminiferous epithelium of testis and may play a role in cell-cell communication during spermatogenensis.
Catalog #C7853-06
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1-3ug/ml
Western Blot: 1-3ug/ml
Immunohistochemistry: 1-3ug/ml on fixed and paraformaldehyde-perfued tissues
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Immunoaffinity chromatgraphy.
ImmunogenSynthetic peptide corresponding to the C-terminal region of mouse connexin 3 protein.
SpecificityRecognizes the ~32kD mouse connexin 31 on Western Blots. The band observed at ~62kD is probably due to dimerization of connexin 31. Several higher bands of unknown origin are observed in Western Blots of mouse skin homogenates. Reactivity has been confirmed with mouse skin homogenate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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