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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 30.3 (CX30.3, Gap Junction Protein, Beta 4 GJB4)

Anti -Connexin 30.3 (CX30.3, Gap Junction Protein, Beta 4 GJB4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #C7853-42
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using ECL
ELISA 1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide C7853-44
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
PurityPurified by immunoaffinity chromatography
ImmunogenA 20 amino acid peptide sequence within the first cytoplasmic domain of rat Cx30.3. The peptide was coupled with KLH (carrier protein) using NH2-terminal Cysteine. The peptide sequence is identical in mouse Cx30.3 and has no significant homology with other connexins.
SpecificityRecognizes rat Cx30.3 (predicted MW: 31kD). Actual crossreactivity of antibodies in various species is not known.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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