| Connexin 30 (Cx-30) is a newly identified member of the connexin gene family. Cx-30 was isolated by screening a genomic mouse library with a rat Cx26 probe (10). The Cx30 protein is most closely related to the Cx26 protein (77% amino acid sequence identity) (10,11). The expression patterns of Cx30 and Cx26 are clearly distinct, specifically, Cx30 is highly expressed in adult skin and brain; however, it cannot be detected in the embryonic and fetal brain (10,11). On the other hand, Cx26 is expressed highly in prenatal brain and decreases after birth. |
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| Catalog # | C7853-60 |
| Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA: 0.1-1.0ug/ml |
| Western Blot (mouse and rat): 1ug/ml; Detects a protein of ~30kD corresponding to the authentic Connexin 30 (Cx30) protein. A Cx30 dimer is not detected although other unidenti ed bands migrating between 70-80kD may be observed. When used to probe Western Blots of rat liver (rat liver does not contain Cx30), several unknown bands are detected at ~110, 53 and 35kD. |
| Immuno uorescence (frozen sections): 1-5ug/ml |
| Immunohistochemistry (frozen): Immunostaining of xed, frozen adult rat brain with C7853-60 produces a typical punctate staining pattern in the globus pallidus/hypothalamus. It is not determined if the antibody is reactive in IHC with mouse samples. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a,k |
| Clone No | 3G102 |
| Host | Mouse |
| Source | Mouse |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to the unique C-terminus of mouse Connexin 30. |
| Specificity | Recognizes mouse Connexin 30. Detects a protein of ~30kD corresponding to the authentic Connexin 30 (Cx30) protein. Species Crossreactivity: rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
